Techniques Involved In DNA Testing

Current Application Of DNA Identity Testing
2017-07-16

D NA testing is a technique that is used to determine the traits of a person by analyzing his DNA sample. This technique is widely used all over the world for things like solving criminal cases, parenting testing, ancestry testing and genetic data assimilation. DNA testing is very simple and accurate.


PCR Analysis

alt textPCR stands for the Polymerase Chain Reactions. The polymerase chain reaction is a technique used to amplify a single segment of DNA to generate numerous copies of a sequence. The DNA sample is denatured and the polynucleotide strand is separated from the whole. Two DNA primers hybridize the two corresponding sites on the nearest DNA. a replication enzyme that is tolerant to high temperatures is used such as Taq polymerase. The process is repeated again and again to make as many copies of the sequence possible. PCR is one of the most accurate and widely used DNA testing technique.

STR Analysis

alt textSTR stands for short tandem repeats. This is similar to the PCR technique but only uses short tandem repeats and short sequences. This method works best because every person has a different number of repeats and comparing the two samples gives accurate results.

DNA family relation analysis- The PCR technique is only used in analyzing the family relationships like maternity, siblings, and parenting. An embryo is formed by the fusion of a sperm and an ovum. The child or the person contains the sequences from both the mother and father. Every person's DNA contains two specific DNA markers: one from the mother and one from the father, that are used to identify an individual by the scientists.

The other techniques involved in the DNA testing are Y-chromosome testing, Mitochondria analysis, and AmpFLP. All these techniques are used depending on the desired result. Each of these techniques is very safe and accurate.

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